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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN14 Monoclonal antibody (67744-1-Ig)

    WB analysis using 67744-1-Ig (same clone as 67744-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67744-1-Ig (PTPN14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PTPN14 Monoclonal antibody (67744-1-Ig)

    IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PTPN14 Monoclonal antibody (67744-1-Ig)

    IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTPN14 Monoclonal antibody (67744-1-Ig)

    IF Staining of HeLa using 67744-1-Ig (same clone as 67744-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTPN14 antibody (67744-1-Ig, Clone: 1B8C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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PTPN14 Monoclonal antibody, PBS Only

PTPN14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67744-1-PBS
Clone No.1B8C8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PTP36, PTP 36, Protein-tyrosine phosphatase pez, PEZ, EC:3.1.3.48

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67744-1-PBS targets PTPN14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTPN14 fusion protein Ag12679 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 14
Calculated molecular weight 1187 aa, 135 kDa
Observed molecular weight150 kDa
GenBank accession numberBC101754
Gene Symbol PTPN14
Gene ID (NCBI) 5784
RRIDAB_2918513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15678
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).