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KD/KO Validated

PTPN2 Polyclonal antibody

Name: PTPN2 Polyclonal antibody
Brand: Proteintech
SKU: 11214-1-AP

PTPN2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11214-1-AP

Synonyms

T-cell protein-tyrosine phosphatase, TC PTP, PTPT, PTPN 2, PTN2

Validation Data Gallery

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AllWBIPIHC

WB analysis of mouse thymus using 11214-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 11214-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11214-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 11214-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11214-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 11214-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 11214-1-AP
IP result of anti-PTPN2 (IP:11214-1-AP, 4ug; Detection:11214-1-AP 1:500) with HL-60 cells lysate 4000ug.
IHC staining of human colon cancer using 11214-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11214-1-AP (PTPN2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 11214-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50.
IHC staining of human colon cancer using 11214-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50.

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Cat No. 11214-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse thymus tissue, HeLa cells, HL-60 cells, NIH/3T3 cells
Positive IP detected in	HL-60 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 5 publications below
IHC	See 7 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

11214-1-AP targets PTPN2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTPN2 fusion protein Ag1709 相同性解析による交差性が予測される生物種
Full Name	protein tyrosine phosphatase, non-receptor type 2
Calculated molecular weight	49 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC016727
Gene symbol	PTPN2
Gene ID (NCBI)	5771
RRID	AB_2173235
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

Protocols

Product Specific Protocols
WB protocol for PTPN2 antibody 11214-1-AP	Download protocol
IHC protocol for PTPN2 antibody 11214-1-AP	Download protocol
IP protocol for PTPN2 antibody 11214-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Oncogene Overexpression of TC-PTP in murine epidermis attenuates skin tumor formation. Authors - Mihwa Kim View Article
human,mouse	WB,IHC	Int Immunopharmacol Hypoxia disrupts the nasal epithelial barrier by inhibiting PTPN2 in chronic rhinosinusitis with nasal polyps Authors - Meiping Zhang KD Validated View Article
mouse	IHC	Int Immunopharmacol Oral squamous cell carcinoma-derived EVs promote tumor progression by regulating inflammatory cytokines and the IL-17A-induced signaling pathway Authors - Ruowei Li View Article
human	WB	Cell Death Dis Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation. Authors - Liang Lu KD Validated View Article
mouse	IHC	Sci Rep Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development. Authors - Hyunseung Lee View Article
human	IF	Infect Immun An Ehrlichia chaffeensis tandem repeat protein interacts with multiple host targets involved in cell signaling, transcriptional regulation, and vesicle trafficking. Authors - Wakeel Abdul A View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPN2 Polyclonal antibody

PTPN2 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of mouse thymus using 11214-1-AP

WB analysis of HL-60 using 11214-1-AP

WB analysis of NIH/3T3 using 11214-1-AP

WB analysis of HL-60 using 11214-1-AP

WB analysis of HeLa using 11214-1-AP

WB analysis of HL-60 using 11214-1-AP

IP experiment of HL-60 using 11214-1-AP

IHC staining of human colon cancer using 11214-1-AP

IHC staining of human colon cancer using 11214-1-AP

IHC staining of human colon cancer using 11214-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Overexpression of TC-PTP in murine epidermis attenuates skin tumor formation.

Hypoxia disrupts the nasal epithelial barrier by inhibiting PTPN2 in chronic rhinosinusitis with nasal polyps

Oral squamous cell carcinoma-derived EVs promote tumor progression by regulating inflammatory cytokines and the IL-17A-induced signaling pathway

Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.

Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development.

An Ehrlichia chaffeensis tandem repeat protein interacts with multiple host targets involved in cell signaling, transcriptional regulation, and vesicle trafficking.