Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | Jurkat cells, Raji cells, Daudi cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85062-2-RR targets PTPN22 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | PTPN22 fusion protein Ag2397 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, non-receptor type 22 (lymphoid) |
| Calculated molecular weight | 82 kDa, 92 kDa |
| Observed molecular weight | 100-110 kDa |
| GenBank accession number | BC017785 |
| Gene Symbol | PTPN22 |
| Gene ID (NCBI) | 26191 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y2R2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPN22 antibody 85062-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |