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KD/KO Validated

PTPN23 Polyclonal antibody

Name: PTPN23 Polyclonal antibody
Brand: Proteintech
SKU: 10472-1-AP

PTPN23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10472-1-AP

Synonyms

KIAA1471, His domain-containing protein tyrosine phosphatase, HD-PTP, HDPTP, HD PTP

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of A375 using 10472-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10472-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10472-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 10472-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10472-1-AP
IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug.
IHC staining of human colon cancer using 10472-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10472-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10472-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10472-1-AP (PTPN23 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 10472-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTPN23 antibody (10472-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Cat No. 10472-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 13 publications below
IHC	See 2 publications below
IF	See 6 publications below
CoIP	See 1 publications below

Product Information

10472-1-AP targets PTPN23 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTPN23 fusion protein Ag0765 相同性解析による交差性が予測される生物種
Full Name	protein tyrosine phosphatase, non-receptor type 23
Calculated molecular weight	179 kDa
Observed molecular weight	179 kDa
GenBank accession number	BC004881
Gene symbol	PTPN23
Gene ID (NCBI)	25930
RRID	AB_2173382
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).

Protocols

Product Specific Protocols
WB protocol for PTPN23 antibody 10472-1-AP	Download protocol
IHC protocol for PTPN23 antibody 10472-1-AP	Download protocol
IF protocol for PTPN23 antibody 10472-1-AP	Download protocol
IP protocol for PTPN23 antibody 10472-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Commun Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation. Authors - Eva Maria Wenzel View Article
human	WB,IHC	Genes Dev Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase. Authors - Siwei Zhang KD Validated View Article
human	IF	EMBO J ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival. Authors - Maja Radulovic View Article
human	WB, IF	Curr Biol UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Authors - Stefani Flavia F View Article
	WB	Curr Biol Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB. Authors - Nazim Ali KD Validated View Article
human	WB	Cell Rep Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9. Authors - Jonathan M Goodwin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPN23 Polyclonal antibody

PTPN23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of A375 using 10472-1-AP

WB analysis of HeLa using 10472-1-AP

WB analysis of HeLa using 10472-1-AP

WB analysis of A375 using 10472-1-AP

IP experiment of HeLa using 10472-1-AP

IHC staining of human colon cancer using 10472-1-AP

IHC staining of human colon cancer using 10472-1-AP

IHC staining of human colon cancer using 10472-1-AP

IF Staining of HepG2 using 10472-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation.

Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.

ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival.

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.

Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.