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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPN6 Polyclonal antibody

PTPN6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24143-1-AP

Synonyms

SHP1, Protein-tyrosine phosphatase 1C, HPTP1C, Hematopoietic cell protein-tyrosine phosphatase, HCPH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN6 Polyclonal antibody (24143-1-AP)

    WB analysis using 24143-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24143-1-AP (PTPN6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PTPN6 Polyclonal antibody (24143-1-AP)

    IHC staining of human ovary cancer using 24143-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24143-1-AP (PTPN6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA431 cells, HL-60 cells, Jurkat cells, K-562 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24143-1-AP targets PTPN6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN6 fusion protein Ag21415 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 6
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC002523
Gene symbol PTPN6
Gene ID (NCBI) 5777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTPN6 antibody 24143-1-APDownload protocol
IHC protocol for PTPN6 antibody 24143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols