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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN6 Polyclonal antibody (24143-1-AP)

    WB analysis using 24143-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24143-1-AP (PTPN6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PTPN6 Polyclonal antibody (24143-1-AP)

    IHC staining of human ovary cancer using 24143-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24143-1-AP (PTPN6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PTPN6 Polyclonal antibody

PTPN6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24143-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

SHP1, Protein-tyrosine phosphatase 1C, HPTP1C, Hematopoietic cell protein-tyrosine phosphatase, HCPH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HL-60 cells, Jurkat cells, K-562 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24143-1-AP targets PTPN6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN6 fusion protein Ag21415 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 6
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC002523
Gene Symbol PTPN6
Gene ID (NCBI) 5777
RRIDAB_3085725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP29350
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTPN6 antibody 24143-1-APDownload protocol
IHC protocol for PTPN6 antibody 24143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols