Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of A549 using 12109-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 12109-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of mouse liver using 12109-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 12109-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 12109-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12109-1-AP
IP result of anti-PTPN9 (IP:12109-1-AP, 4ug; Detection:12109-1-AP 1:300) with HEK-293 cells lysate 1800ug.
IHC staining of human liver cancer using 12109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PTPN9 Polyclonal antibody

PTPN9 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12109-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

MEG2, PTPase MEG2, PTPMEG2, PTPN9

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, Transfected HEK-293 cells, mouse liver tissue, mouse pancreas tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

12109-1-AP targets PTPN9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTPN9 fusion protein Ag2754 相同性解析による交差性が予測される生物種
Full Name	protein tyrosine phosphatase, non-receptor type 9
Calculated molecular weight	593 aa, 68 kDa
Observed molecular weight	68-70 kDa
GenBank accession number	BC010863
Gene Symbol	PTPN9
Gene ID (NCBI)	5780
RRID	AB_2877826
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43378
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177). PTPN9 can be detected double bands (PMID: 22394684). PTPN9 was located in cytoplasm and nucleus in human breast cancer (PMID: 23418360).

Protocols

Product Specific Protocols
WB protocol for PTPN9 antibody 12109-1-AP	Download protocol
IHC protocol for PTPN9 antibody 12109-1-AP	Download protocol
IP protocol for PTPN9 antibody 12109-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids MicroRNA-126 Regulates Angiogenesis and Neurogenesis in a Mouse Model of Focal Cerebral Ischemia. Authors - Meijie Qu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 12109-1-AP

WB analysis of mouse pancreas using 12109-1-AP

WB analysis of mouse liver using 12109-1-AP

WB analysis using 12109-1-AP

WB analysis of Transfected HEK-293 using 12109-1-AP