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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPRD Polyclonal antibody

PTPRD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27941-1-AP

Synonyms

PTPD, Protein-tyrosine phosphatase delta, HPTPD, HPTP DELTA, HPTP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPRD Polyclonal antibody (27941-1-AP)

    WB analysis using 27941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27941-1-AP (PTPRD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTPRD Polyclonal antibody (27941-1-AP)

    WB analysis using 27941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27941-1-AP (PTPRD antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PTPRD Polyclonal antibody (27941-1-AP)

    IHC staining of human liver using 27941-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27941-1-AP (PTPRD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PTPRD Polyclonal antibody (27941-1-AP)

    IHC staining of human liver using 27941-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27941-1-AP (PTPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, human placenta tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27941-1-AP targets PTPRD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRD fusion protein Ag27689 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, D
Calculated molecular weight 1912 aa, 215 kDa
Observed molecular weight75 kDa, 175 kDa
GenBank accession numberBC106714
Gene symbol PTPRD
Gene ID (NCBI) 5789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPRD is a receptor-type protein tyrosine phosphatase that plays significant roles in various biological processes, including cell adhesion, synaptic function, and signal transduction. PTPRD is highly expressed in the brain, followed by the kidney, ovary, placenta, and intestine. Within the brain, it is found in specific regions, suggesting a role in neurological functions. PTPRD has emerged as a potential therapeutic target for various brain phenotypes and disorders. Its genetic variations have been associated with nervous system phenotypes, and the discovery of the first small molecule inhibitor of PTPRD phosphatase has opened new avenues for therapeutic intervention in addiction and possibly other PTPRD-associated disorders. Additionally, PTPRD is considered a tumor-suppressor gene that is down-regulated in hepatocellular carcinoma, suggesting its role in cancer progression and potential as a therapeutic target. The protein can recognize 175 kDa full length and 75 kDa mature cleavage product (PMID: 19074898, PMID: 25113440).

Protocols

Product Specific Protocols
WB protocol for PTPRD antibody 27941-1-APDownload protocol
IHC protocol for PTPRD antibody 27941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols