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Validation Data Gallery

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  • Western Blot (WB) analysis of pig spleen tissue using PTPRH Monoclonal antibody (68635-1-Ig)

    WB analysis of pig spleen using 68635-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 68635-1-Ig (PTPRH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PTPRH Monoclonal antibody (68635-1-Ig)

    WB analysis of A431 using 68635-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68635-1-Ig (PTPRH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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PTPRH Monoclonal antibody

PTPRH Monoclonal Antibody for WB, ELISA
Cat No. 68635-1-Ig
Clone No.3C6E8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

SAP1, SAP 1, R PTP H, 3C6E8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig spleen tissue, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68635-1-Ig targets PTPRH in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTPRH fusion protein Ag17351 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, H
Calculated molecular weight 1115 aa, 122 kDa
Observed molecular weight200-210 kDa
GenBank accession numberBC111716
Gene Symbol PTPRH
Gene ID (NCBI) 5794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9HD43
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPRH, also known as SAP-1 (Stomach Cancer-Associated Protein-Tyrosine Phosphatase-1), is a member of the protein tyrosine phosphatase (PTP) family. These enzymes regulate cellular signaling by dephosphorylating tyrosine residues on proteins, counterbalancing kinase activity (PMID: 11278335). PTPRH is classified as a receptor-type PTP, characterized by an extracellular domain, a transmembrane region, and intracellular catalytic domains. In colorectal, gastric, and hepatocellular cancers, PTPRH is often upregulated and linked to poor prognosis (PMID: 12101188). Paradoxically, it may suppress tumorigenesis in other contexts by stabilizing cell adhesion. Overexpression in certain cancers suggests utility as a diagnostic or prognostic marker. The molecular weight of PTPRH is 122 kDa, it is reported that it can form a dimer with a molecular weight of 210 kDa (PMID:15850787).

Protocols

Product Specific Protocols
WB protocol for PTPRH antibody 68635-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols