Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
67000-1-PBS targets PTPRO in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig samples.
| Tested Reactivity | Human, Pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PTPRO fusion protein Ag8284 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, receptor type, O |
| Calculated molecular weight | 138 kDa |
| Observed molecular weight | 160 kDa, 180-220 kDa |
| GenBank accession number | BC035960 |
| Gene Symbol | PTPRO |
| Gene ID (NCBI) | 5800 |
| RRID | AB_2882317 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16827 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).