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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPRO Monoclonal antibody, PBS Only

PTPRO Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F2B4

Cat no : 67000-1-PBS

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

Validation Data Gallery

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  • Western Blot (WB) analysis of pig kidney tissue using PTPRO Monoclonal antibody (67000-1-Ig)

    WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using PTPRO Monoclonal antibody (67000-1-Ig)

    WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PTPRO Monoclonal antibody (67000-1-Ig)

    IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PTPRO Monoclonal antibody (67000-1-Ig)

    IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PTPRO Monoclonal antibody (67000-1-Ig)

    IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PTPRO Monoclonal antibody (67000-1-Ig)

    IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using PTPRO Monoclonal antibody (67000-1-Ig)

    IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using PTPRO Monoclonal antibody (67000-1-Ig)

    IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67000-1-PBS targets PTPRO in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PTPRO fusion protein Ag8284 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, O
Calculated molecular weight 138 kDa
Observed molecular weight 160 kDa, 180-220 kDa
GenBank accession numberBC035960
Gene symbol PTPRO
Gene ID (NCBI) 5800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).