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PTPRS Polyclonal antibody

PTPRS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29415-1-AP

Synonyms

Receptor-type tyrosine-protein phosphatase sigma, Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPRS Polyclonal antibody (29415-1-AP)

    WB analysis using 29415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29415-1-AP (PTPRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PTPRS Polyclonal antibody (29415-1-AP)

    WB analysis of HEK-293 using 29415-1-AP

    WB result of PTPRS antibody (29415-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRS transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PTPRS Polyclonal antibody (29415-1-AP)

    WB analysis using 29415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29415-1-AP (PTPRS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTPRS Polyclonal antibody (29415-1-AP)

    IHC staining of mouse brain using 29415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29415-1-AP (PTPRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTPRS Polyclonal antibody (29415-1-AP)

    IHC staining of mouse brain using 29415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29415-1-AP (PTPRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29415-1-AP targets PTPRS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRS fusion protein Ag30152 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, S
Calculated molecular weight 217 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_002850
Gene symbol PTPRS
Gene ID (NCBI) 5802
RRIDAB_2918301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPRS, also known as R-PTP-sigma or PTPσ, is a member of the protein tyrosine phosphatase (PTP) family (PMID: 8524829). PTPs dephosphorylate phosphotyrosyl residues in proteins that are phosphorylated by protein-tyrosine kinases (PTKs) (PMID: 20838449). PTPs are essential regulators of signal transduction pathways and play important roles in regulating many cellular processes in conjunction with PTKs (PMID: 7834742). PTPRS contains an extracellular region, a single transmembrane segment and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. Full-length PTPRS is 217 kDa and has been reported to be proteolytically cleaved into a 140-kDa N-terminal subunit containing extracellular and transmembrane domains and a C-terminal subunit (PMID: 29915291). This antibody raised against 825-1000 aa of human PTPRS detects endogenous PTPRS with an apparent molecular weight of 140 kDa.

Protocols

Product Specific Protocols
WB protocol for PTPRS antibody 29415-1-APDownload protocol
IHC protocol for PTPRS antibody 29415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols