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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTPRT Polyclonal antibody

PTPRT Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25473-1-AP

Synonyms

KIAA0283, PTPRT, R PTP T, RPTP rho, RPTPrho

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTPRT Polyclonal antibody (25473-1-AP)

    IHC staining of mouse brain using 25473-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25473-1-AP (PTPRT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PTPRT Polyclonal antibody (25473-1-AP)

    IHC staining of mouse brain using 25473-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25473-1-AP (PTPRT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25473-1-AP targets PTPRT in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, T
Calculated molecular weight 164 kDa
GenBank accession numberNM_007050
Gene symbol PTPRT
Gene ID (NCBI) 11122
RRIDAB_3085797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPRT is a member of the type IIB receptor-type protein tyrosine phosphatase family, linked to signal transduction, cell adhesion and neurite extension. PTPRT plays a role in regulation of synaptic formation and neuronal development. It regulates synaptic function and neuronal development through interactions with neuronal adhesion molecules and the dephosphorylation of synaptic molecules. PTPRT is mainly expressed in nervous system (PMID:19816407, PMID: 25748173).

Protocols

Product Specific Protocols
IHC protocol for PTPRT antibody 25473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols