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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPRU Polyclonal antibody (23786-1-AP)

    WB analysis using 23786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23786-1-AP (PTPRU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PTPRU Polyclonal antibody (23786-1-AP)

    IF Staining of U-251 using 23786-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PTPRU antibody (23786-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PTPRU Polyclonal antibody

PTPRU Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23786-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PTP, PCP2, PCP 2, hPTP J, GLEPP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23786-1-AP targets PTPRU in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRU fusion protein Ag20667 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, U
Calculated molecular weight 1446 aa, 162 kDa
Observed molecular weight170 kDa
GenBank accession numberBC146655
Gene Symbol PTPRU
Gene ID (NCBI) 10076
RRIDAB_3669416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ92729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPRU (protein tyrosine phosphatase, receptor type, U) is also named as PCP-2,PTP-J, PTP pi, PTP-RO and R-PTP-psi. Protein tyrosine phosphatase plays a crucial role in the regulation of signal transduction during diverse cell functions, including cell activation, proliferation, differentiation, survival, and metabolic homeostasis. PTPases are classified into two groups, membrane-spanning receptor-type (R-PTPases) and cytosolictype. The human PTPRU cDNA clone encodes a polypeptide of 1,439 amino acids and the predicted molecular mass of the PTPRU protein is 162 kDa. It has reported that SCF induced tyrosine phosphorylation of the full-length PTPRU (190 kDa) and the proteolytic forms of PTPRU (100 and 80 kD). A non-tyrosine phosphorylated 73 kDa band was detected in addition to those of 190, 90, and 80 kDa. (PMID: 10397721).

Protocols

Product Specific Protocols
WB protocol for PTPRU antibody 23786-1-APDownload protocol
IF protocol for PTPRU antibody 23786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols