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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTRF Polyclonal antibody (30086-1-AP)

    WB analysis using 30086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30086-1-AP (PTRF antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PTRF Polyclonal antibody (30086-1-AP)

    IHC staining of human stomach using 30086-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30086-1-AP (PTRF antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PTRF Polyclonal antibody (30086-1-AP)

    IF Staining of HepG2 using 30086-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PTRF antibody (30086-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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PTRF Polyclonal antibody

PTRF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30086-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FKSG13, Cavin-1, Cavin 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HT-1080 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30086-1-AP targets PTRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTRF fusion protein Ag32786 相同性解析による交差性が予測される生物種
Full Name polymerase I and transcript release factor
Calculated molecular weight 43 kDa
Observed molecular weight55 kDa
GenBank accession numberBC008849
Gene Symbol PTRF
Gene ID (NCBI) 284119
RRIDAB_2935513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NZI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transcription of RNA Pol I, which involves pausing of transcription by TTF1, and dissociation of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55 kDa. (PMID: 11139612 )

Protocols

Product Specific Protocols
WB protocol for PTRF antibody 30086-1-APDownload protocol
IHC protocol for PTRF antibody 30086-1-APDownload protocol
IF protocol for PTRF antibody 30086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols