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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTRH2 Polyclonal antibody

PTRH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 51006-2-AP

Synonyms

BIT1, CGI 147, peptidyl tRNA hydrolase 2, PTH 2, PTH2, PTRH2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis using 51006-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HEK-293 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of HEK-293 using 51006-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of Jurkat using 51006-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HepG2 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of HepG2 using 51006-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    IF Staining of HEK-293 using 51006-2-AP

    Immunofluorescent analysis of HEK-293 cells using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51006-2-AP targets PTRH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTRH2 fusion protein Ag0839 相同性解析による交差性が予測される生物種
Full Name peptidyl-tRNA hydrolase 2
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC006807
Gene symbol PTRH2
Gene ID (NCBI) 51651
RRIDAB_2173280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTRH2 antibody 51006-2-APDownload protocol
IHC protocol for PTRH2 antibody 51006-2-APDownload protocol
IF protocol for PTRH2 antibody 51006-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols