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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTRH2 Polyclonal antibody, PBS Only

PTRH2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51006-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis using 51006-2-AP (same clone as 51006-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of HEK-293 using 51006-2-AP (same clone as 51006-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of Jurkat using 51006-2-AP (same clone as 51006-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of HepG2 using 51006-2-AP (same clone as 51006-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP (same clone as 51006-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP (same clone as 51006-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP (same clone as 51006-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    IF Staining of HEK-293 using 51006-2-AP (same clone as 51006-2-PBS)

    Immunofluorescent analysis of HEK-293 cells using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51006-2-PBS targets PTRH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTRH2 fusion protein Ag0839 相同性解析による交差性が予測される生物種
Full Name peptidyl-tRNA hydrolase 2
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC006807
Gene symbol PTRH2
Gene ID (NCBI) 51651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.