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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTRH2 Polyclonal antibody, PBS Only

PTRH2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51006-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis using 51006-2-AP (same clone as 51006-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of HEK-293 using 51006-2-AP (same clone as 51006-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of Jurkat using 51006-2-AP (same clone as 51006-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    WB analysis of HepG2 using 51006-2-AP (same clone as 51006-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP (same clone as 51006-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP (same clone as 51006-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTRH2 Polyclonal antibody (51006-2-AP)

    IHC staining of human prostate cancer using 51006-2-AP (same clone as 51006-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PTRH2 Polyclonal antibody (51006-2-AP)

    IF Staining of HEK-293 using 51006-2-AP (same clone as 51006-2-PBS)

    Immunofluorescent analysis of HEK-293 cells using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51006-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51006-2-PBS targets PTRH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTRH2 fusion protein Ag0839 相同性解析による交差性が予測される生物種
Full Name peptidyl-tRNA hydrolase 2
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC006807
Gene symbol PTRH2
Gene ID (NCBI) 51651
RRIDAB_2173280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.