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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PUF-A,KIAA0020 Monoclonal antibody (67941-1-Ig)

    WB analysis using 67941-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HSC-T6 cells using PUF-A,KIAA0020 Monoclonal antibody (67941-1-Ig)

    WB analysis of HSC-T6 using 67941-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using PUF-A,KIAA0020 Monoclonal antibody (67941-1-Ig)

    WB analysis of PC-12 using 67941-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PUF-A,KIAA0020 Monoclonal antibody (67941-1-Ig)

    WB analysis of NIH/3T3 using 67941-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using PUF-A,KIAA0020 Monoclonal antibody (67941-1-Ig)

    WB analysis of 4T1 using 67941-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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PUF-A,KIAA0020 Monoclonal antibody

PUF-A,KIAA0020 Monoclonal Antibody for WB, ELISA
Cat No. 67941-1-Ig
Clone No.1G4B7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

HLA HA8, KIAA0020, PEN, PUF6, PUF-A, PUM3, XTP5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, k-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67941-1-Ig targets PUF-A,KIAA0020 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PUF-A,KIAA0020 fusion protein Ag31324 相同性解析による交差性が予測される生物種
Full Name KIAA0020
Calculated molecular weight 648 aa, 74 kDa
Observed molecular weight68-70 kDa
GenBank accession numberBC016137
Gene Symbol PUF-A/KIAA0020
Gene ID (NCBI) 9933
RRIDAB_2918693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15397
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PUF-A,KIAA0020 antibody 67941-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols