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  • Featured Product
  • KD/KO Validated

PUF60 Polyclonal antibody

PUF60 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10810-1-AP

Synonyms

FBP interacting repressor, FIR, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of HeLa using 10810-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of rat testis using 10810-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of mouse testis using 10810-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of HepG2 using 10810-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human colon cancer using 10810-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human breast cancer using 10810-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human colon cancer using 10810-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human breast cancer using 10810-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10810-1-AP targets PUF60 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PUF60 fusion protein Ag1053 相同性解析による交差性が予測される生物種
Full Name poly-U binding splicing factor 60KDa
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC008875
Gene symbol PUF60
Gene ID (NCBI) 22827
RRIDAB_2173583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.

Protocols

Product Specific Protocols
WB protocol for PUF60 antibody 10810-1-APDownload protocol
IHC protocol for PUF60 antibody 10810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

USP22 regulates cell proliferation by deubiquitinating the transcriptional regulator FBP1.

Authors - Atanassov Boyko S BS
View Article
mouseWB

Cell Rep

CLOCK regulates Drp1 mRNA stability and mitochondrial homeostasis by interacting with PUF60.

Authors - Lirong Xu
  • KD Validated
View Article