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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PUF60 Monoclonal antibody, PBS Only

PUF60 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3A1

Cat no : 68533-1-PBS

Synonyms

FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PUF60 Monoclonal antibody (68533-1-Ig)

    WB analysis using 68533-1-Ig (same clone as 68533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PUF60 Monoclonal antibody (68533-1-Ig)

    WB analysis of mouse testis using 68533-1-Ig (same clone as 68533-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68533-1-PBS targets PUF60 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PUF60 fusion protein Ag33930 相同性解析による交差性が予測される生物種
Full Name poly-U binding splicing factor 60KDa
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC008875
Gene symbol PUF60
Gene ID (NCBI) 22827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.