Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15982-1-AP targets PUM1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PUM1 fusion protein Ag8790 相同性解析による交差性が予測される生物種 |
| Full Name | pumilio homolog 1 (Drosophila) |
| Calculated molecular weight | 1186 aa, 126 kDa |
| GenBank accession number | BC013398 |
| Gene Symbol | PUM1 |
| Gene ID (NCBI) | 9698 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PUM1, also named as PUMH1, PUML1 and Pumilio 1, is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains one PUM-HD domain and eight pumilio repeats. It is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. It is strongly downregulated in keratinocytes upon UVB irradiation. The human PUM1 protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. It may be required to support proliferation and self-renewal of stem cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human PUM1.
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics. |