Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
11586-1-AP targets PUM2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PUM2 fusion protein Ag2179 相同性解析による交差性が予測される生物種 |
| Full Name | pumilio homolog 2 (Drosophila) |
| Calculated molecular weight | 114 kDa |
| Observed molecular weight | 95 kDa, 140 kDa |
| GenBank accession number | BC024218 |
| Gene Symbol | PUM2 |
| Gene ID (NCBI) | 23369 |
| RRID | AB_2284639 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TB72 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PUM2 (Human Pumilio homology protein 2), a member of the PUF protein family, binds to 3′ untranslated regions (3′ UTRs) of mRNA and to repress gene expression by controlling cytoplasmic polyadenylation and affecting mRNA translation [PMID:12459267]. When translationally dormant, PUM2, CPEB (CPE-binding protein) and Maskin form a complex on maternal cyclin B1 mRNA, precluding the formation of the active translation initiation complex [PMID:15687499]. PUM2 has been found to play a role in the mammalian nervous system, in particular in regulating morphology, arborization and excitability of neuronal dendrites [PMID:22016787]. The calculated molecular weight of PUM2 is 114 kDa, but the modified PUM2 protein is about 140 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PUM2 antibody 11586-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a.
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