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PURA Monoclonal antibody

PURA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C6G4

Cat no : 67305-1-Ig

Synonyms

PUR ALPHA, PUR1, PURA, PURALPHA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PURA Monoclonal antibody (67305-1-Ig)

    WB analysis using 67305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PURA Monoclonal antibody (67305-1-Ig)

    WB analysis of HepG2 using 67305-1-Ig

    WB result of PURA antibody (67305-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PURA transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using PURA Monoclonal antibody (67305-1-Ig)

    WB analysis using 67305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PURA Monoclonal antibody (67305-1-Ig)

    WB analysis using 67305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, pig brain tissue, rat brain tissue, mouse brain tissue, L02 cells, LNCaP cells, human placenta tissue, rat cerebellum tissue, mouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67305-1-Ig targets PURA in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PURA fusion protein Ag12164 相同性解析による交差性が予測される生物種
Full Name purine-rich element binding protein A
Calculated molecular weight 322 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC036087
Gene symbol PURA
Gene ID (NCBI) 5813
RRIDAB_2882567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.

Protocols

Product Specific Protocols
WB protocol for PURA antibody 67305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols