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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PXMP3 Polyclonal antibody

PXMP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22163-1-AP

Synonyms

PAF 1, PAF1, Peroxin 2, Peroxisomal membrane protein 3, Peroxisome assembly factor 1, Peroxisome biogenesis factor 2, PEX2, PMP3, PMP35, PXMP3, RING finger protein 72, RNF72

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    WB analysis of mouse liver using 22163-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    WB analysis of mouse liver using 22163-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PXMP3 Polyclonal antibody (22163-1-AP)

    WB analysis of HeLa using 22163-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    WB analysis of mouse brain using 22163-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    IHC staining of human liver using 22163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    IHC staining of human liver using 22163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

22163-1-AP targets PXMP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PXMP3 fusion protein Ag17815 相同性解析による交差性が予測される生物種
Full Name peroxisomal membrane protein 3, 35kDa
Calculated molecular weight 305 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC000661
Gene symbol PXMP3
Gene ID (NCBI) 5828
RRIDAB_2879014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PXMP3 antibody 22163-1-APDownload protocol
IHC protocol for PXMP3 antibody 22163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses.

Authors - Anu S Nath
View Article