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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    WB analysis of mouse liver using 22163-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    WB analysis of mouse liver using 22163-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PXMP3 Polyclonal antibody (22163-1-AP)

    WB analysis of HeLa using 22163-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    WB analysis of mouse brain using 22163-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    IHC staining of human liver using 22163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PXMP3 Polyclonal antibody (22163-1-AP)

    IHC staining of human liver using 22163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 40x lens).

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PXMP3 Polyclonal antibody

PXMP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22163-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

PAF 1, PAF1, Peroxin 2, Peroxisomal membrane protein 3, Peroxisome assembly factor 1, Peroxisome biogenesis factor 2, PEX2, PMP3, PMP35, PXMP3, RING finger protein 72, RNF72

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

22163-1-AP targets PXMP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PXMP3 fusion protein Ag17815 相同性解析による交差性が予測される生物種
Full Name peroxisomal membrane protein 3, 35kDa
Calculated molecular weight 305 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC000661
Gene Symbol PXMP3
Gene ID (NCBI) 5828
RRIDAB_2879014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28328
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PXMP3 antibody 22163-1-APDownload protocol
IHC protocol for PXMP3 antibody 22163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses.

Authors - Anu S Nath
View Article