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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PXR Monoclonal antibody (67912-1-Ig)

    WB analysis using 67912-1-Ig (same clone as 67912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PXR Monoclonal antibody (67912-1-Ig)

    WB analysis of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PXR Monoclonal antibody (67912-1-Ig)

    WB analysis of COLO 320 using 67912-1-Ig (same clone as 67912-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PXR Monoclonal antibody (67912-1-Ig)

    IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PXR Monoclonal antibody (67912-1-Ig)

    IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PXR Monoclonal antibody (67912-1-Ig)

    FC experiment of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PXR (67912-1-Ig, Clone:1G3A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

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PXR Monoclonal antibody, PBS Only

PXR Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67912-1-PBS
Clone No.1G3A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

NR1I2, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, ONR1, BXR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67912-1-PBS targets PXR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PXR fusion protein Ag7991 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 1, group I, member 2
Calculated molecular weight 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC017304
Gene Symbol PXR
Gene ID (NCBI) 8856
RRIDAB_2918667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75469
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID: 22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].