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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASC/TMS1 Recombinant antibody

ASC/TMS1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240908D5

Cat no : 83858-3-RR

Synonyms

Pycard, PYD and CARD domain containing, masc, CARD5, Asc

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PYCARD Recombinant antibody (83858-3-RR)

    WB analysis using 83858-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83858-3-RR (PYCARD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83858-3-RR

    Affinity and Kinetic Characterization of 83858-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83858-3-RR against Mouse PYCARD were performed. The affinity constant is 3.58 nM.

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Tested Applications

Positive WB detected inJ774A.1 cells, mouse lung tissue, mouse thymus tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83858-3-RR is ASC/TMS1 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ASC/TMS1 fusion protein Ag32519 相同性解析による交差性が予測される生物種
Full Name PYD and CARD domain containing
Calculated molecular weight21KD
Observed molecular weight21-25 kDa
GenBank accession numberNM_023258
Gene symbol Pycard
Gene ID (NCBI) 66824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).

Protocols

Product Specific Protocols
WB protocol for ASC/TMS1 antibody 83858-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols