• Featured Product
  • KD/KO Validated

PYCR1 Polyclonal antibody

PYCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13108-1-AP

Synonyms

PIG45, P5CR 1, P5CR, P5C reductase 1, P5C


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, human brain tissue, mouse embryo tissue, mouse liver tissue, NIH/3T3 cells, HT-1080 cells,mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13108-1-AP targets PYCR1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR1 fusion protein Ag3764 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase 1
Calculated molecular weight 319 aa, 33.8 kDa
Observed molecular weight 33 kDa, 35 kDa
GenBank accession numberBC022244
Gene symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_2174878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PYCR1 antibody 13108-1-APDownload protocol
IHC protocol for PYCR1 antibody 13108-1-APDownload protocol
IF protocol for PYCR1 antibody 13108-1-APDownload protocol
IP protocol for PYCR1 antibody 13108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nature

Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.

Authors - Fabricio Loayza-Puch
  • KO Validated
humanWB

Nature

Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.

Authors - Possemato Richard R
zebrafishWB,IF

Nat Genet

Mutations in PYCR1 cause cutis laxa with progeroid features.

Authors - Reversade Bruno B
mouseWB

Cell Metab

Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.

Authors - Sukru Anil Dogan
humanIHC

J Hepatol

Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.

Authors - Zhaobing Ding
human,mouseWB,IF

Neuron

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Authors - Nathalie Escande-Beillard

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hyeonju (Verified Customer) (05-13-2024)

Too many non-specific bands are captured. ? I'm curious what the band is under pycr1 in lanes 6-9. Can you send me information about other proteins that react with the pycr1 antibody(if you have information about this)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver tissue
PYCR1 Antibody Western Blot validation (1:1000 dilution) in mouse liver tissue (Cat no:13108-1-AP)
FH

Daniel (Verified Customer) (02-05-2020)

The antibody stained the glomerulus of the mouse kidney.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney
PYCR1 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:13108-1-AP)