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PYCR1 Polyclonal antibody

Name: PYCR1 Polyclonal antibody
Brand: Proteintech
SKU: 13108-1-AP
Rating: 3.50 (2 reviews)

PYCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 13108-1-AP

Synonyms

PIG45, P5CR 1, P5CR, P5C reductase 1, P5C

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 13108-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 13108-1-AP
WB result of PYCR1 antibody (13108-1-AP, 1:5000) with si-Control and si-PYCR1 transfected HeLa cells.
WB analysis of mouse liver using 13108-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13108-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse embryo using 13108-1-AP
mouse embryo tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 13108-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 13108-1-AP
IP result of anti-PYCR1 (IP:13108-1-AP, 3ug; Detection:13108-1-AP 1:700) with mouse brain tissue lysate 4000ug.
IHC staining of human prostate cancer using 13108-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 13108-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 13108-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13108-1-AP (PYCR1 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 13108-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PYCR1 antibody (13108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of MCF-7 using 13108-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PYCR1 antibody (13108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 13108-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells, human brain tissue, mouse embryo tissue, mouse liver tissue, NIH/3T3 cells, HT-1080 cells，mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 45 publications below
IHC	See 11 publications below
IF	See 13 publications below
IP	See 7 publications below
CoIP	See 1 publications below

Product Information

13108-1-AP targets PYCR1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PYCR1 fusion protein Ag3764 相同性解析による交差性が予測される生物種
Full Name	pyrroline-5-carboxylate reductase 1
Calculated molecular weight	319 aa, 33.8 kDa
Observed molecular weight	33 kDa, 35 kDa
GenBank accession number	BC022244
Gene symbol	PYCR1
Gene ID (NCBI)	5831
RRID	AB_2174878
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PYCR1 antibody 13108-1-AP	Download protocol
IHC protocol for PYCR1 antibody 13108-1-AP	Download protocol
IF protocol for PYCR1 antibody 13108-1-AP	Download protocol
IP protocol for PYCR1 antibody 13108-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Nature Tumour-specific proline vulnerability uncovered by differential ribosome codon reading. Authors - Fabricio Loayza-Puch KO Validated View Article
human	WB	Nature Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Authors - Possemato Richard R View Article
zebrafish	WB,IF	Nat Genet Mutations in PYCR1 cause cutis laxa with progeroid features. Authors - Reversade Bruno B View Article
mouse	WB	Cell Metab Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy. Authors - Sukru Anil Dogan View Article
human	IHC	J Hepatol Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis. Authors - Zhaobing Ding View Article
human,mouse	WB,IF	Neuron Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2. Authors - Nathalie Escande-Beillard View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Hyeonju (Verified Customer) (05-13-2024)

Too many non-specific bands are captured. ? I'm curious what the band is under pycr1 in lanes 6-9. Can you send me information about other proteins that react with the pycr1 antibody(if you have information about this)

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: mouse liver tissue

PYCR1 Antibody Western Blot validation (1:1000 dilution) in mouse liver tissue (Cat no:13108-1-AP)

Daniel (Verified Customer) (02-05-2020)

The antibody stained the glomerulus of the mouse kidney.

Applications: Immunofluorescence,
Primary Antibody Dilution: 1:200
Cell Tissue Type: Mouse kidney

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYCR1 Polyclonal antibody

PYCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 13108-1-AP

WB analysis of HeLa cells using 13108-1-AP

WB analysis of mouse liver using 13108-1-AP

WB analysis of human brain using 13108-1-AP

WB analysis of mouse embryo using 13108-1-AP

WB analysis of NIH/3T3 using 13108-1-AP

IP experiment of mouse brain using 13108-1-AP

IHC staining of human prostate cancer using 13108-1-AP

IHC staining of human prostate cancer using 13108-1-AP

IHC staining of human prostate cancer using 13108-1-AP

IF Staining of HepG2 using 13108-1-AP

IF Staining of MCF-7 using 13108-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.

Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.

Mutations in PYCR1 cause cutis laxa with progeroid features.

Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.

Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Reviews