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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYCR1-specific Polyclonal antibody

PYCR1-specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20962-1-AP

Synonyms

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using PYCR1-specific Polyclonal antibody (20962-1-AP)

    WB analysis of Raji using 20962-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PYCR1-specific Polyclonal antibody (20962-1-AP)

    WB analysis of human brain using 20962-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PYCR1-specific Polyclonal antibody (20962-1-AP)

    WB analysis of Raji using 20962-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PYCR1-specific Polyclonal antibody (20962-1-AP)

    WB analysis of Raji using 20962-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using PYCR1-specific Polyclonal antibody (20962-1-AP)

    IP experiment of Raji using 20962-1-AP

    IP result of anti-PYCR1-specific (IP:20962-1-AP, 4ug; Detection:20962-1-AP 1:500) with Raji cells lysate 1440ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PYCR1-specific Polyclonal antibody (20962-1-AP)

    IHC staining of human colon cancer using 20962-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20962-1-AP (PYCR1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PYCR1-specific Polyclonal antibody (20962-1-AP)

    IHC staining of human colon cancer using 20962-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20962-1-AP (PYCR1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inRaji cells, human brain tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20962-1-AP targets PYCR1-specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase 1
Calculated molecular weight 33 kDa
Observed molecular weight 34 kDa
GenBank accession numberNM_006907
Gene symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_10638486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody is specific to PYCR1.

Protocols

Product Specific Protocols
WB protocol for PYCR1-specific antibody 20962-1-APDownload protocol
IHC protocol for PYCR1-specific antibody 20962-1-APDownload protocol
IP protocol for PYCR1-specific antibody 20962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Authors - Simon Schwörer
View Article
human,mouseWB

EMBO Mol Med

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy

Authors - Nneka Southwell
View Article