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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PYCR1 Polyclonal antibody (22150-1-AP)

    WB analysis of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PYCR1 Polyclonal antibody (22150-1-AP)

    WB analysis of COLO 320 using 22150-1-AP (same clone as 22150-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PYCR1 Polyclonal antibody (22150-1-AP)

    IP experiment of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    IP result of anti-PYCR1 (IP:22150-1-AP, 4ug; Detection:22150-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PYCR1 Polyclonal antibody (22150-1-AP)

    IF Staining of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22150-1-AP (PYCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PYCR1 Polyclonal antibody, PBS Only

PYCR1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 22150-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22150-1-PBS targets PYCR1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR1 fusion protein Ag17745 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase 1
Calculated molecular weight 319 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC022244
Gene Symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_11182280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32322
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody may have cross reaction to PYCR2 due to the high homology.