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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYCR1 Monoclonal antibody, PBS Only (Detector)

PYCR1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2G1

Cat no : 66510-1-PBS

Synonyms

P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PYCR1 Monoclonal antibody (66510-1-Ig)

    WB analysis using 66510-1-Ig (same clone as 66510-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66510-1-Ig (PYCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1 hour. This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PYCR1 Monoclonal antibody (66510-1-Ig)

    IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PYCR1 Monoclonal antibody (66510-1-Ig)

    IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PYCR1 Monoclonal antibody (66510-1-Ig)

    IF Staining of HeLa using 66510-1-Ig (same clone as 66510-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR1 antibody (66510-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50786-1

    Cytometric bead array standard curve of MP50786-1

    Cytometric bead array standard curve of MP50786-1, PYCR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66510-2-PBS. Detection antibody: 66510-1-PBS. Standard:Ag17919. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66510-1-PBS targets PYCR1 as part of a matched antibody pair:

MP50786-1: 66510-2-PBS capture and 66510-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PYCR1 fusion protein Ag17919 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase 1
Calculated molecular weight 319 aa, 33.8 kDa
Observed molecular weight 33 kDa, 35 kDa
GenBank accession numberBC022244
Gene symbol PYCR1
Gene ID (NCBI) 5831
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.