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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYCR2 Monoclonal antibody

PYCR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G7D9

Cat no : 68488-1-Ig

Synonyms

FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PYCR2 Monoclonal antibody (68488-1-Ig)

    WB analysis using 68488-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PYCR2 Monoclonal antibody (68488-1-Ig)

    WB analysis using 68488-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Tested Applications

Positive WB detected inJurkat cells, LNCaP cells, K-562 cells, THP-1 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68488-1-Ig targets PYCR2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PYCR2 fusion protein Ag10923 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase family, member 2
Calculated molecular weight 320 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC020553
Gene symbol PYCR2
Gene ID (NCBI) 29920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PYCR2 antibody 68488-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols