Validation Data Gallery
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Tested Applications
| Positive WB detected in | MDA-MB-231 cells, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
16008-1-AP targets PYCRL in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PYCRL fusion protein Ag8716 相同性解析による交差性が予測される生物種 |
| Full Name | pyrroline-5-carboxylate reductase-like |
| Calculated molecular weight | 274 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC007993 |
| Gene Symbol | PYCRL |
| Gene ID (NCBI) | 65263 |
| RRID | AB_3669228 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53H96 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proline conversion into P5C is coupled to the P5C reduction into proline, catalyzed by P5C reductase. This enzyme has three isoforms: PYCR1/PYCR2, which are specific for the mitochondrial compartment and the PYCRL present in cytosol. The conversion of P5C to proline, and its transport between mitochondria and cytosol, are also coupled with glucose metabolism by the pentose phosphate pathway.(PMID: 35163433)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PYCRL antibody 16008-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.
| ||
Vet Microbiol Host factor cyclophilin B affects Orf virus replication by interacting with viral ORF058 protein. |