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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PYGL Polyclonal antibody

PYGL Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 55429-1-AP

Synonyms

GSD6, phosphorylase, glycogen, liver, PYGL

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HepG2 cells using PYGL Polyclonal antibody (55429-1-AP)

    IP experiment of HepG2 using 55429-1-AP

    IP result of anti-PYGL (IP:55429-1-AP, 4ug; Detection:55429-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PYGL Polyclonal antibody (55429-1-AP)

    IHC staining of human skeletal muscle using 55429-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55429-1-AP (PYGL antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PYGL Polyclonal antibody (55429-1-AP)

    IHC staining of human skeletal muscle using 55429-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55429-1-AP (PYGL antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PYGL Polyclonal antibody (55429-1-AP)

    IF Staining of HepG2 using 55429-1-AP

    Immunofluorescent analysis of HepG2 cells using 55429-1-AP (PYGL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells, mouse liver
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55429-1-AP targets PYGL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phosphorylase, glycogen, liver
Calculated molecular weight 97 kDa
Observed molecular weight 97-105 kDa
GenBank accession numberNM_002863
Gene symbol PYGL
Gene ID (NCBI) 5836
RRIDAB_11182599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PYGL belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). This antibody is specific to PYGL. It has no cross reaction to PYGM and PYGB.

Protocols

Product Specific Protocols
WB protocol for PYGL antibody 55429-1-APDownload protocol
IHC protocol for PYGL antibody 55429-1-APDownload protocol
IF protocol for PYGL antibody 55429-1-APDownload protocol
IP protocol for PYGL antibody 55429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols