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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Pan Acetylation Monoclonal antibody (66289-1-Ig)

    WB analysis of HeLa using 66289-1-Ig (same clone as 66289-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66289-1-Ig (Pan Acetylation Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66289-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Pan Acetylation Monoclonal antibody (66289-1-Ig)

    IF Staining of HeLa using 66289-1-Ig (same clone as 66289-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66289-1-Ig (Pan Acetylation antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66289-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Pan Acetylation Monoclonal antibody (66289-1-Ig)

    IF Staining of MDCK using 66289-1-Ig (same clone as 66289-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 66289-1-Ig (Pan Acetylation antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66289-1-PBS in a different storage buffer formulation.

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Pan Acetylation Monoclonal antibody, PBS Only

Pan Acetylation Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66289-1-PBS
Clone No.1D5F2

Host / Isotype

Mouse / IgG1

Reactivity

chemical compound

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Acetylation Lysine, Acetylation, Ac-lysine

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66289-1-PBS targets Pan Acetylation in WB, IF/ICC, Indirect ELISA applications and shows reactivity with chemical compound samples.

Tested Reactivity chemical compound
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Pan Acetylation
Observed molecular weight 17 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Acetylation and De-acetylation are the important reaction in epigenetic. Many proteins, especially Histone, are with Acetylated of Lysine. This antibody is used to detect the Acetylation of Lysine. The 17 kDa in our detection is Acetylated Histone.