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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Pan-cadherin Polyclonal antibody (31313-1-AP)

    WB analysis using 31313-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Pan-cadherin Polyclonal antibody (31313-1-AP)

    IHC staining of mouse colon using 31313-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Pan-cadherin Polyclonal antibody (31313-1-AP)

    IHC staining of mouse colon using 31313-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Pan-cadherin Polyclonal antibody (31313-1-AP)

    IHC staining of mouse heart using 31313-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Pan-cadherin Polyclonal antibody (31313-1-AP)

    IHC staining of mouse heart using 31313-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Pan-cadherin Polyclonal antibody (31313-1-AP)

    IF Staining of MCF-7 using 31313-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Pan-cadherin antibody (31313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Pan-cadherin Polyclonal antibody

Pan-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31313-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

E-cadherin, CDH1, CD324, CAM 120/80, Cadherin-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, C6 cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inmouse colon tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31313-1-AP targets Pan-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTG 相同性解析による交差性が予測される生物種
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight97 kDa
Observed molecular weight120-130 kDa
GenBank accession numberNM_004360
Gene Symbol E-cadherin
Gene ID (NCBI) 999
RRIDAB_3669943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP12830
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in tissue morphogenesis and the maintenance of normal tissue architecture (PMID: 23885883). The classic cadherin family members include E-, N-, P-, R-, and B-cadherins. The classic cadherins have ectodomains composed of five extracellular cadherin (EC) repeats, a single transmembrane region, and a cytoplasmic domain. Structural characterization indicated that the known cadherins are glycoproteins ranging in their apparent molecular weights between 120 and 140 kDa (PMID: 2077036). This polyclonal antibody is raised against a synthetic peptide corresponding to a conserved region of human E-Cadherin.

Protocols

Product Specific Protocols
WB protocol for Pan-cadherin antibody 31313-1-APDownload protocol
IHC protocol for Pan-cadherin antibody 31313-1-APDownload protocol
IF protocol for Pan-cadherin antibody 31313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols