Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
29312-1-AP targets Parvalbumin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Parvalbumin fusion protein Ag30189 相同性解析による交差性が予測される生物種 |
| Full Name | parvalbumin |
| Calculated molecular weight | 12 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | NM_002854 |
| Gene Symbol | Parvalbumin |
| Gene ID (NCBI) | 5816 |
| RRID | AB_2918280 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20472 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| IHC protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| IF protocol for Parvalbumin antibody 29312-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Mol Neurosci PGC-1α regulates critical period onset/closure, mediating cortical plasticity | ||
Transl Androl Urol Whole body vibration training promotes proprioceptive pathway for the treatment of stress urinary incontinence in rats | ||
Mol Neurobiol Oxidative Stress-mediated Loss of Hippocampal Parvalbumin Interneurons Contributes to Memory Precision Decline After Acute Sleep Deprivation | ||
Cell Rep Methods A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution | ||
STAR Protoc Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID | ||
Sci Rep Identification of mouse soleus muscle proteins altered in response to changes in gravity loading |