Validation Data Gallery

Filter: AllWBIHCIF-PIF-Fro

WB analysis using 29312-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 29312-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 29312-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse cerebellum using 29312-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 29312-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 29312-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 29312-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 29312-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 29312-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 29312-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse cerebellum using 29312-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 29312-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 29312-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse cerebellum using 29312-1-AP
Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 29312-1-AP
Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat cerebellum using 29312-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat cerebellum using 29312-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum tissue using 29312-1-AP
Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:1500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University)

Parvalbumin Polyclonal antibody

Parvalbumin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 29312-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

PVALB, Parvalbumin alpha

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue
Positive IHC detected in	mouse cerebellum tissue, mouse brain tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse cerebellum tissue, mouse brain tissue, rat cerebellum tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 2 publications below
IF	See 10 publications below

Product Information

29312-1-AP targets Parvalbumin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag30189 Product name: Recombinant human PVALB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-110 aa of NM_002854 Sequence: SMTDLLNAEDIKKAVGAFSATDSFDHKKFFQMVGLKKKSADDVKKVFHMLDKDKSGFIEEDELGFILKGFSPDARDLSAKETKMLMAAGDKDGDGKIGVDEFSTLVAES 相同性解析による交差性が予測される生物種
Full Name	parvalbumin
Calculated molecular weight	12 kDa
Observed molecular weight	15 kDa
GenBank accession number	NM_002854
Gene Symbol	Parvalbumin
Gene ID (NCBI)	5816
RRID	AB_2918280
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20472
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).

Protocols

Product Specific Protocols
IF protocol for Parvalbumin antibody 29312-1-AP	Download protocol
IHC protocol for Parvalbumin antibody 29312-1-AP	Download protocol
WB protocol for Parvalbumin antibody 29312-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IF	Int J Mol Sci Remodeling of Perineuronal Nets in the Striato-Cortical Axis in L-DOPA-Induced Dyskinesia Rat Model. Authors - Nedime Tugce Bilbay View Article
mouse	WB,IHC,IF	Front Mol Neurosci PGC-1α regulates critical period onset/closure, mediating cortical plasticity Authors - Wei-Jun Zhang View Article
rat	IF	Physiol Res High-Intensity Interval Training Increases Osteoarthritis-Associated Pain-Sensitive Threshold Through Reduction of Perineuronal Nets of the Medial Prefrontal Cortex in Rats Authors - X Zhang View Article
rat	IHC	Transl Androl Urol Whole body vibration training promotes proprioceptive pathway for the treatment of stress urinary incontinence in rats Authors - Chengyu Zhou View Article
mouse	WB	FASEB J Loss of STARD7 Impairs Mitochondrial Phospholipid Homeostasis and Contributes to Mitochondrial Myopathy. Authors - Yasuhiro Horibata View Article
mouse	IF	Acta Pharm Sin B The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy. Authors - Jiali Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 29312-1-AP

WB analysis using 29312-1-AP

WB analysis using 29312-1-AP

IHC staining of mouse cerebellum using 29312-1-AP

IHC staining of mouse cerebellum using 29312-1-AP

IHC staining of rat cerebellum using 29312-1-AP

IHC staining of rat cerebellum using 29312-1-AP

IHC staining of mouse brain using 29312-1-AP

IHC staining of mouse brain using 29312-1-AP

IF Staining of mouse cerebellum using 29312-1-AP

IF Staining of mouse cerebellum using 29312-1-AP

IF Staining of mouse brain using 29312-1-AP

IF Staining of mouse brain using 29312-1-AP

IF Staining of mouse cerebellum using 29312-1-AP

IF Staining of mouse cerebellum using 29312-1-AP

IF Staining of rat cerebellum using 29312-1-AP

IF Staining of rat cerebellum using 29312-1-AP

IF Staining of mouse cerebellum tissue using 29312-1-AP

Parvalbumin Polyclonal antibody

Parvalbumin Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Remodeling of Perineuronal Nets in the Striato-Cortical Axis in L-DOPA-Induced Dyskinesia Rat Model.

PGC-1α regulates critical period onset/closure, mediating cortical plasticity

High-Intensity Interval Training Increases Osteoarthritis-Associated Pain-Sensitive Threshold Through Reduction of Perineuronal Nets of the Medial Prefrontal Cortex in Rats

Whole body vibration training promotes proprioceptive pathway for the treatment of stress urinary incontinence in rats

Loss of STARD7 Impairs Mitochondrial Phospholipid Homeostasis and Contributes to Mitochondrial Myopathy.

The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy.

Able™

Able^™