• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Perforin Recombinant antibody, PBS Only (Detector)

Perforin Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241009C10

Cat no : 83977-1-PBS

Synonyms

P1, Lymphocyte pore-forming protein, HPLH2, FLH2, Cytolysin

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Perforin Recombinant antibody (83977-1-RR)

    WB analysis using 83977-1-RR (same clone as 83977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83977-1-RR (Perforin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83977-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00872-1

    Cytometric bead array standard curve of MP00872-1

    Cytometric bead array standard curve of MP00872-1, Perforin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83977-2-PBS. Detection antibody: 83977-1-PBS. Standard: Ag6060. Range: 0.781-100 ng/mL.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83977-1-PBS targets Perforin as part of a matched antibody pair:

MP00872-1: 83977-2-PBS capture and 83977-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Perforin fusion protein Ag6060 相同性解析による交差性が予測される生物種
Full Name perforin 1 (pore forming protein)
Calculated molecular weight 61 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC063043
Gene symbol Perforin
Gene ID (NCBI) 5551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Perforin (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)