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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Pericentrin Polyclonal antibody

Pericentrin Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27084-1-AP

Synonyms

KEN, Kendrin, KIAA0402, MOPD2, PCN, PCNT, PCNT2, PCNTB, PCTN2, pericentrin, Pericentrin B, SCKL4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Pericentrin Polyclonal antibody (27084-1-AP)

    IHC staining of human thyroid cancer using 27084-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27084-1-AP (Pericentrin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Pericentrin Polyclonal antibody (27084-1-AP)

    IHC staining of human thyroid cancer using 27084-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27084-1-AP (Pericentrin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Pericentrin Polyclonal antibody (27084-1-AP)

    IF Staining of HeLa using 27084-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (27084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Pericentrin Polyclonal antibody (27084-1-AP)

    IF Staining of HeLa using 27084-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (27084-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27084-1-AP targets Pericentrin in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pericentrin fusion protein Ag25888 相同性解析による交差性が予測される生物種
Full Name pericentrin
Calculated molecular weight 378 kDa
GenBank accession numberAK093923
Gene symbol Pericentrin
Gene ID (NCBI) 5116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2).

Protocols

Product Specific Protocols
IHC protocol for Pericentrin antibody 27084-1-APDownload protocol
IF protocol for Pericentrin antibody 27084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols