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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using Periostin Monoclonal antibody (66491-1-Ig)

    WB analysis of BxPC-3 using 66491-1-Ig (same clone as 66491-1-PBS)

    BxPC-3, SGC-7901 and ROS1728 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using Periostin Monoclonal antibody (66491-1-Ig)

    WB analysis of Neuro-2a using 66491-1-Ig (same clone as 66491-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Periostin Monoclonal antibody (66491-1-Ig)

    WB analysis of human placenta using 66491-1-Ig (same clone as 66491-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IHC staining of human stomach using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IHC staining of human colon cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IHC staining of human colon cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Periostin Monoclonal antibody (66491-1-Ig)

    IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Periostin Monoclonal antibody, PBS Only

Periostin Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66491-1-PBS
Clone No.1A11A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Osteoblast-specific factor 2, Osteoblast specific factor 2, OSF-2, OSF2, OSF 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66491-1-PBS targets Periostin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Periostin fusion protein Ag14487 相同性解析による交差性が予測される生物種
Full Name periostin, osteoblast specific factor
Calculated molecular weight 93 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC106710
Gene Symbol Periostin
Gene ID (NCBI) 10631
RRIDAB_2881856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15063
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot, PMID: 21997759).