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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-4EBP1 (Thr37) Recombinant antibody (81812-4-RR)

    WB analysis using 81812-4-RR

    Non-treated HEK-293T cells, phosphatase inhibitor treated HEK-293T cells and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81812-4-RR (Phospho-4EBP1 (Thr37) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Phospho-4EBP1 (Thr37) Recombinant antibody

Phospho-4EBP1 (Thr37) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81812-4-RR
Clone No.240535G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293T cells, λ phosphatase treated HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81812-4-RR targets Phospho-4EBP1 (Thr37) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4E binding protein 1
Calculated molecular weight 118 aa, 12 kDa
Observed molecular weight 15-20 kDa
GenBank accession numberBC004459
Gene Symbol EIF4EBP1
Gene ID (NCBI) 1978
RRIDAB_3670507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13541
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)

Protocols

Product Specific Protocols
WB protocol for Phospho-4EBP1 (Thr37) antibody 81812-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols