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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-ACC1 (Ser79) Polyclonal antibody, PBS Only

Phospho-ACC1 (Ser79) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29119-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-ACC1 (Ser79) Polyclonal antibody (29119-1-AP)

    WB analysis using 29119-1-AP (same clone as 29119-1-PBS)

    Non-treated SH-SY5Y, phosphatase inhibitor treated and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control. This data was developed using the same antibody clone with 29119-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-ACC1 (Ser79) Polyclonal antibody (29119-1-AP)

    WB analysis using 29119-1-AP (same clone as 29119-1-PBS)

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29119-1-AP (Phospho-ACC1 (Ser79) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29119-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29119-1-PBS targets Phospho-ACC1 (Ser79) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight 2383 aa, 275 kDa
Observed molecular weight 250 kDa
GenBank accession numberBC137287
Gene symbol Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)