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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-ACC1 (Ser79) Recombinant antibody

Phospho-ACC1 (Ser79) Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240235G11

Cat no : 80281-2-RR

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-ACC1 (Ser79) Recombinant antibody (80281-2-RR)

    WB analysis of NIH/3T3 using 80281-2-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80281-2-RR (Phospho-ACC1 (Ser79) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-ACC1 (Ser79) Recombinant antibody (80281-2-RR)

    IF Staining of HeLa using 80281-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-ACC1 (Ser79) antibody (80281-2-RR, Clone: 240235G11 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Tested Applications

Positive WB detected inλ phosphatase treated NIH/3T3 cells
Positive IF detected inCalyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80281-2-RR targets Phospho-ACC1 (Ser79) in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight 2383 aa, 275 kDa
Observed molecular weight 250-270 kDa
GenBank accession numberBC137287
Gene symbol ACACA
Gene ID (NCBI) 31
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)

Protocols

Product Specific Protocols
WB protocol for Phospho-ACC1 (Ser79) antibody 80281-2-RRDownload protocol
IF protocol for Phospho-ACC1 (Ser79) antibody 80281-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols