Validation Data Gallery
Tested Applications
| Positive WB detected in | PMA treated HL-60 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
30266-1-AP targets Phospho-ALK (Tyr1604) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | anaplastic lymphoma receptor tyrosine kinase |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC157090 |
| Gene Symbol | ALK |
| Gene ID (NCBI) | 238 |
| RRID | AB_2935424 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UM73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The human ALK appears to exist as a 140 kDa protein, as well as the 220 kDa full-length ALK species and the 140 kDa ALK species is thought to be the result of a cleavage within the extracellular region of full-length ALK. The 140 kDa ALK protein is phosphorylated in response to activation of ALK(PMID: 19459784).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ALK (Tyr1604) antibody 30266-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Med Chem Distinct Amino Acid-Based PROTACs Target Oncogenic Kinases for Degradation in Non-Small Cell Lung Cancer (NSCLC) |