Phospho-AMPK Beta 1 (Ser182) Recombinant antibody
Phospho-AMPK Beta 1 (Ser182) Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240628B1
Cat no : 83924-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, λ phosphatase treated HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
83924-1-RR targets Phospho-AMPK Beta 1 (Ser182) in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | protein kinase, AMP-activated, beta 1 non-catalytic subunit |
Calculated molecular weight | 38 kDa |
Observed molecular weight | 38 kDa |
GenBank accession number | BC001007 |
Gene symbol | PRKAB1 |
Gene ID (NCBI) | 5564 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880). This antibody recognizes phosphorylated AMPK Beta 1.
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-AMPK Beta 1 (Ser182) antibody 83924-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv Bioenergetic-active exosomes for cartilage regeneration and homeostasis maintenance |