Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal antibody
Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28790-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Anisomycin treated NIH/3T3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28790-1-AP targets Phospho-ATF2 (Thr71)/ATF7 (Thr53) in WB, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | activating transcription factor 2 |
Calculated molecular weight | 209 aa, 23 kDa |
Observed molecular weight | 60-70 kDa |
GenBank accession number | BC026175 |
Gene symbol | ATF2 |
Gene ID (NCBI) | 1386 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Stress and growth factors activate ATF2 and ATF7 mainly via sequential phosphorylation of two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated protein kinases (MAPK), phosphorylate ATF2 on Thr71 and ATF7 on Thr53, resulting in transcriptional activation. The antibody recognizes ATF2 phosphorylation sites Thr71 and ATF7 phosphorylation sites Thr53.
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-ATF2 (Thr71)/ATF7 (Thr53) antibody 28790-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |