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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Beta Catenin (Ser33) Polyclonal antibody, PBS Only

Phospho-Beta Catenin (Ser33) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28772-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Beta Catenin (Ser33) Polyclonal antibody (28772-1-AP)

    WB analysis using 28772-1-AP (same clone as 28772-1-PBS)

    Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28772-1-AP (Phospho-Beta Catenin (Ser33) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28772-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-Beta Catenin (Ser33) Polyclonal antibody (28772-1-AP)

    WB analysis using 28772-1-AP (same clone as 28772-1-PBS)

    Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28772-1-AP (Phospho-Beta Catenin (Ser33) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28772-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28772-1-PBS targets Phospho-Beta Catenin (Ser33) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight90-100 kDa
GenBank accession numberBC058926
Gene symbol Beta Catenin
Gene ID (NCBI) 1499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Beta Catenin/β-catenin, also known as CTNNB1, is a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The N-terminal (1-49 amino acids) of β-catenin is the key region for its stability and the degradation by ubiquitin-proteasome system, and phosphorylation of β-catenin at the N-terminal Ser33, Ser37, Thr41, and Ser45 promotes its ubiquitination and degradation (PMID: 31930681). Phosphorylated β-catenin is targeted for ubiquitination-dependent proteolysis.