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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Beta Catenin (Ser33) Recombinant antibody, PBS Only

Phospho-Beta Catenin (Ser33) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K1

Cat no : 80067-1-PBS

Synonyms

Beta catenin, Catenin beta 1, beta-Catenin, B-catenin, b cat

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Beta Catenin (Ser33) Recombinant antibody (80067-1-RR)

    WB analysis using 80067-1-RR (same clone as 80067-1-PBS)

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (Phospho-Beta Catenin (Ser33)antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-Beta Catenin (Ser33) Recombinant antibody (80067-1-RR)

    WB analysis using 80067-1-RR (same clone as 80067-1-PBS)

    Non-treated HT-29 and Calyculin A treated treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (CTNNB1 antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using Phospho-Beta Catenin (Ser33) Recombinant antibody (80067-1-RR)

    FC experiment of PC-3 using 80067-1-RR (same clone as 80067-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-Beta Catenin (Ser33) (80067-1-RR, Clone:3K1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80067-1-PBS targets Phospho-Beta Catenin (Ser33) in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight90 kDa
GenBank accession numberBC058926
Gene symbol Beta Catenin
Gene ID (NCBI) 1499
RRIDAB_2918861
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .