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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Beta Catenin (Ser33) Recombinant antibody, PBS Only

Phospho-Beta Catenin (Ser33) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K1

Cat no : 80067-1-PBS

Synonyms

Beta catenin, Catenin beta 1, beta-Catenin, B-catenin, b cat

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Beta Catenin (Ser33) Recombinant antibody (80067-1-RR)

    WB analysis using 80067-1-RR (same clone as 80067-1-PBS)

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (Phospho-Beta Catenin (Ser33)antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-Beta Catenin (Ser33) Recombinant antibody (80067-1-RR)

    WB analysis using 80067-1-RR (same clone as 80067-1-PBS)

    Non-treated HT-29 and Calyculin A treated treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80067-1-RR (CTNNB1 antibody) at dilution of 1:10000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with beta tubulin (66240-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using Phospho-Beta Catenin (Ser33) Recombinant antibody (80067-1-RR)

    FC experiment of PC-3 using 80067-1-RR (same clone as 80067-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-Beta Catenin (Ser33) (80067-1-RR, Clone:3K1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80067-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80067-1-PBS targets Phospho-Beta Catenin (Ser33) in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight90 kDa
GenBank accession numberBC058926
Gene symbol Beta Catenin
Gene ID (NCBI) 1499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .