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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-Beta Catenin (Ser552) Polyclonal antibody

Phospho-Beta Catenin (Ser552) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28778-1-AP

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser552), Phospho-CTNNB1 (Ser552), PRO2286, β-Catenin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Beta Catenin (Ser552) Polyclonal antibody (28778-1-AP)

    WB analysis using 28778-1-AP

    Untreated and Forskolin treated cells were subjected to SDS PAGE followed by western blot with 28778-1-AP (Phospho-Beta Catenin (Ser552) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Phospho-Beta Catenin (Ser552) Polyclonal antibody (28778-1-AP)

    IF Staining of MCF-7 using 28778-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Beta Catenin (Ser552) antibody (28778-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inForskolin treated A431 cells, HSC-T6 cells, Forskolin treated HSC-T6 cells, HT-29 cells, Forskolin treated HT-29 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28778-1-AP targets Phospho-Beta Catenin (Ser552) in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight86-100 kDa
GenBank accession numberBC058926
Gene symbol Beta Catenin
Gene ID (NCBI) 1499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers(PMID: 22617422, PMID: 18334222). Both AMPK and AKT phosphorylates β-catenin at Ser 552. The phosphorylation of β-catenin at Ser552 can stabilize β-catenin, enhance its nuclear accumulation and increase its transcriptional activity(PMID: 2865606, PMID: 20361929).

Protocols

Product Specific Protocols
WB protocol for Phospho-Beta Catenin (Ser552) antibody 28778-1-APDownload protocol
IF protocol for Phospho-Beta Catenin (Ser552) antibody 28778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols