Phospho-Beta Catenin (Ser552) Polyclonal antibody
Phospho-Beta Catenin (Ser552) Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 28778-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Forskolin treated A431 cells, HSC-T6 cells, Forskolin treated HSC-T6 cells, HT-29 cells, Forskolin treated HT-29 cells |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28778-1-AP targets Phospho-Beta Catenin (Ser552) in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
Calculated molecular weight | 781 aa, 86 kDa |
Observed molecular weight | 86-100 kDa |
GenBank accession number | BC058926 |
Gene symbol | Beta Catenin |
Gene ID (NCBI) | 1499 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers(PMID: 22617422, PMID: 18334222). Both AMPK and AKT phosphorylates β-catenin at Ser 552. The phosphorylation of β-catenin at Ser552 can stabilize β-catenin, enhance its nuclear accumulation and increase its transcriptional activity(PMID: 2865606, PMID: 20361929).
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-Beta Catenin (Ser552) antibody 28778-1-AP | Download protocol |
IF protocol for Phospho-Beta Catenin (Ser552) antibody 28778-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |