Phospho-CHEK2 (Thr68) Polyclonal antibody

Phospho-CHEK2 (Thr68) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29012-1-AP

Synonyms

CDS1, CHEK2, CHK2, HuCds1, LFS2, PP1425, RAD53


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Tested Applications

Positive WB detected inMMS treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

29012-1-AP targets Phospho-CHEK2 (Thr68) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name CHK2 checkpoint homolog (S. pombe)
Calculated molecular weight 61 kDa
Observed molecular weight65 kDa
GenBank accession numberBC004207
Gene symbol CHEK2
Gene ID (NCBI) 11200
RRIDAB_2918230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)

Protocols

Product Specific Protocols
WB protocol for Phospho-CHEK2 (Thr68) antibody 29012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Prolif

eIF3a-PPP2R5A-mediated ATM/ATR dephosphorylation is essential for irinotecan-induced DNA damage response.

Authors - Chao Mei
humanWB

Front Oncol

ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway

Authors - Xin Wen
humanWB

Research (Wash D C)

Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer

Authors - Yangyang Li
humanWB

Cell Death Dis

PI3K/mTOR inhibitors promote G6PD autophagic degradation and exacerbate oxidative stress damage to radiosensitize small cell lung cancer

Authors - Huan Deng
humanWB

Front Oncol

Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway

Authors - Zhili Xia