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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-CREB1 (Ser133) Recombinant antibody (81871-1-RR)

    WB analysis of HEK-293 using 81871-1-RR (same clone as 81871-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:20000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta-tubulin antibody as loading control. This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Phospho-CREB1 (Ser133) Recombinant antibody (81871-1-RR)

    IHC staining of human prostate cancer using 81871-1-RR (same clone as 81871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Phospho-CREB1 (Ser133) Recombinant antibody (81871-1-RR)

    IHC staining of human prostate cancer using 81871-1-RR (same clone as 81871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Phospho-CREB1 (Ser133) Recombinant antibody (81871-1-RR)

    IHC staining of mouse colon using 81871-1-RR (same clone as 81871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Phospho-CREB1 (Ser133) Recombinant antibody (81871-1-RR)

    IHC staining of mouse colon using 81871-1-RR (same clone as 81871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

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Phospho-CREB1 (Ser133) Recombinant antibody, PBS Only

Phospho-CREB1 (Ser133) Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81871-1-PBS
Clone No.5H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

CREB1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81871-1-PBS targets Phospho-CREB1 (Ser133) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 1
Calculated molecular weight 341 aa, 35 kDa
Observed molecular weight 35-46 kDa
GenBank accession numberBC010636
Gene Symbol CREB1
Gene ID (NCBI) 1385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16220
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)