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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-CRKL (Tyr207) Recombinant antibody (84590-1-RR)

    WB analysis using 84590-1-RR (same clone as 84590-1-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84590-1-RR (Phospho-CRKL (Tyr207) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84590-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using Phospho-CRKL (Tyr207) Recombinant antibody (84590-1-RR)

    FC experiment of K-562 using 84590-1-RR (same clone as 84590-1-PBS)

    1X10^6 K-562 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-CRKL (Tyr207) Recombinant antibody (84590-1-RR, Clone:240881B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84590-1-PBS in a different storage buffer formulation.

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Phospho-CRKL (Tyr207) Recombinant antibody, PBS Only

Phospho-CRKL (Tyr207) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84590-1-PBS
Clone No.240881B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

CRKL, 240881B3

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84590-1-PBS targets Phospho-CRKL (Tyr207) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Calculated molecular weight 34 kDa
Observed molecular weight39 kDa
GenBank accession numberBC043500
Gene Symbol CRKL
Gene ID (NCBI) 1399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP46109
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776)