Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells |
| Positive FC (Intra) detected in | λ phosphatase treated K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84590-1-RR targets Phospho-CRKL (Tyr207) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-crk sarcoma virus CT10 oncogene homolog (avian)-like |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC043500 |
| Gene Symbol | CRKL |
| Gene ID (NCBI) | 1399 |
| RRID | AB_3672086 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P46109 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-CRKL (Tyr207) antibody 84590-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |